PBMC Cryopreservation Medium
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For the cryopreservation of PB- and CB-derived mononuclear cells and buffy coats
Class I medical device filing
GMP-compliant management with fully PRC Pharmacopoeia ingredients
Ready-to-use and long-term cryopreservation
High-viability and stable population
Cryopreservation
Collect the mononuclear cells from peripheral blood or cord blood, centrifuge the cell (500×g,5minutes,RT) and remove the supernatant.
Resuspend cell pellet with proper solution (saline,culture medium or DPBS) ,followed by cell counting and cell survival rate detection.A viability of over 90% before cryopreservation is preferred. Then centrifuge (500×g,5 minutes,RT) and remove the supernatant.
Resuspend cell pellet using the product to reach a cell density of 1×10⁶-1×108/mL and a freezing volume of 0.5-1.5 mL/tube.If using a cryopreservation bagforlarge volume cryopreservation,please contact the technical team to obtain the programed cooling curve. Users can also add autologous plasma, serum,or HSA according to experimental needs to improve post-thaw viability.
Transfer the cryovial to a-80℃ freezer.If long-term freezing is required,transfer to liquid nitrogen after 12hours.
Thawing
1. After removing from liquid nitrogen,immediately immerse the cryovial into a 37℃ water bath,stir to rapidly thaw the cells within 2 minutes.
2. Transfer thawed cells to a conical tube,slowly add 9 times volume of proper diluent.
3. Equilibrate by gently invert the tube for 10 times.
4. Centrifuge (500×g,5 minutes,RT) and remove the supernatant.
5. Resuspend cell pellet with proper media/solution.
6. Perform cell count test if needed.
7. Proceed for downstream application.
Viability test
Add thawed cells to 4 times the volume of complete culture medium,transfer to a centrifuge tube,and centrifuge for 5 minutes.Discard the supernatant,resuspend the cells using DPBS or complete culture medium,and then test the cell survival rate.
Ordering Information of PBMC Medium
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CellStore Biotechnology Co., Ltd. ("CellStore") is an innovative enterprise specialized in the R&D and production of cryopreservation medium for bio-samples. We have built a leading technology platform for cryopreservation of biological samples.
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